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. 2019 Apr 17;12:2891–2902. doi: 10.2147/OTT.S201228

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© 2019 Liu et al.

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The oncogenic effect of Nr5a2 is mediated by activating the Wnt/β-catenin signaling pathway. (A) The effect of Nr5a2 knockdown on the activity of Wnt/β-catenin signaling pathway was assessed by dual-luciferase reporter assays in AGS cells. The results are expressed as mean ± SD of three independent experiments. (B) The expression levels of β-catenin, Wnt3a, and the Wnt/β-catenin signaling pathway target proteins c-Myc and CyclinD1 in control and Nr5a2 knockdown cells were detected by western blotting. (C) AGS-shNr5a2 cells were treated with or without 10 mM LiCl (an activator of the Wnt/β-catenin signaling pathway), and the expression levels of Wnt/β-catenin signaling pathway-related proteins were examined by western blotting. (D) The effect of LiCl on cell proliferation in Nr5a2-downregulated AGS cells was evaluated by a CCK-8 assay. The differences between the shNr5a2+ PBS and shNr5a2+ LiCl groups were analyzed byunpaired t-tests. (E) The effect of LiCl on cell migration in Nr5a2-downregulated AGS cells was evaluated by Transwell assay. The results are expressed as mean ± SD of three independent experiments. *P<0.05, **P<0.01, ***P<0.001. NC, negative control.