Figure 5.

Cultured oligodendrocytes and the effects of hypothermia on oligodendrocytes differentiation. A, Almost all of the cultured oligodendrocytes were stained by NG2 but not MBP at 0 d. B, Protocol for oligodendrocytes culture and hypothermia administration. C, Quantitative RT‐PCR was performed to detect NG2 and MBP mRNA changes in the different groups. Hypothermia promotes oligodendrocytes differentiation, as manifested by decreased NG2 mRNA and increased MBP mRNA after oligodendrocytes differentiation was induced by T3 for 8 d. Data are expressed as fold change versus 37°C group. *P < 0.05. D, Representative immunofluorescence images of stage‐specific oligodendrocytes markers by NG2 and MBP at 8 d after culture in each group. Scale bar: 50 μm