Table 2. Strategies for the detection of RNA methylation.

Method type	Input quantity	Resolution	Stoichiometry information	Sequence data	Types of modification detected
Radioisotope incorporation	Medium	Low	Medium	Nil	All
Thin-layer chromatography	Low	Low	High	Low	All
Mass spectrometry	High	Medium	Medium	Low	All
Differential enzyme/chemical–RNA interactions	High	High	Low	Maintained	Ribose
Bisulphite RNA sequencing	High	High	Low	Maintained	m5C
Antibody-based sequencing	High	High	Low	Maintained	Base methylations
Big data	None	Low	Very low	Maintained	All

A brief summary of the commonly used methods for detecting modifications to nucleotides in RNA.