Table 1.

MicroRNA sequencing analysis of the mouse prefrontal cortex (PFC) astrocytes in response to acute phencyclidine (PCP) treatment

MATURE miRNA ID	PRE‐ACC	MATURE‐SEQ	PCP vs Control fold change
PCP vs Control 2.0‐fold change Upregulated miRNAs
mmu‐miR‐212‐5p	MI0000696	ACCUUGGCUCUAGACUGCUUACU	4.06
mmu‐miR‐127‐3p	MI0000154	UCGGAUCCGUCUGAGCUUGGCU	2.90
mmu‐miR‐183‐5p	MI0000225	UAUGGCACUGGUAGAAUUCACU	2.48
mmu‐miR‐298‐5p	MI0000398	GGCAGAGGAGGGCUGUUCUUCCC	2.46
mmu‐miR‐381‐3p	MI0000798	UAUACAAGGGCAAGCUCUCUGU	2.42
mmu‐miR‐338‐3p	MI0000619	UCCAGCAUCAGUGAUUUUGUUG	2.40
mmu‐let‐7d‐3p	MI0000405	CUAUACGACCUGCUGCCUUUCU	2.38
mmu‐miR‐132‐3p	MI0000158	UAACAUGCUACAGCCAUGGUCG	2.22
mmu‐miR‐130a‐3p	MI0000156	CAGUGCAAUGUUAAAAGGGCAU	2.19
mmu‐miR‐744‐5p	MI0004124	UGCGGGGCUAGGGCUAACAGCA	2.17
mmu‐miR‐330‐5p	MI0000607	UCUCUGGGCCUGUGUCUUAGGC	2.13
mmu‐miR‐335‐3p	MI0000817	UUUUUCAUUAUUGCUCCUGACC	2.06
mmu‐miR‐29c‐3p	MI0000577	UAGCACCAUUUGAAAUCGGUUA	2.04
mmu‐miR‐181a‐1‐3p	MI0000697	ACCAUCGACCGUUGAUUGUACC	2.04
mmu‐miR‐872‐5p	MI0005549	AAGGUUACUUGUUAGUUCAGG	2.00
PCP vs Control 2.0‐fold change Downregulated miRNAs
mmu‐miR‐143‐3p	MI0000257	UGAGAUGAAGCACUGUAGCUC	0.26
mmu‐miR‐15a‐5p	MI0000564	UAGCAGCACAUAAUGGUUUGUG	0.45
mmu‐miR‐1968‐5p	MI0009965	UGCAGCUGUUAAGGAUGGUGGACU	0.50
mmu‐miR‐582‐3p	MI0006127	UAACCUGUUGAACAACUGAAC	0.50

GFAP‐GFP transgenic mouse brains were rapidly removed 30 min after subcutaneous injection with PCP (4 mg/kg) or saline as control. GFP positive astrocytes were sorted by flow cytometry from prefrontal cortex. RNA was extracted from sorted astrocytes and used to prepare the miRNA sequencing libraries. The libraries were captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 36 cycles on Illumina HiSeq following the manufacturer's instructions. Trimmed reads were alignment to the miRBase pre‐miRNAs. miRNA read counts were normalized as tag counts per million miRNA alignments. Differentially expressed miRNAs (PCP vs Control >2.0 fold changes) were presented in the table.