Table 1.
MATURE miRNA ID | PRE‐ACC | MATURE‐SEQ | PCP vs Control fold change |
---|---|---|---|
PCP vs Control 2.0‐fold change Upregulated miRNAs | |||
mmu‐miR‐212‐5p | MI0000696 | ACCUUGGCUCUAGACUGCUUACU | 4.06 |
mmu‐miR‐127‐3p | MI0000154 | UCGGAUCCGUCUGAGCUUGGCU | 2.90 |
mmu‐miR‐183‐5p | MI0000225 | UAUGGCACUGGUAGAAUUCACU | 2.48 |
mmu‐miR‐298‐5p | MI0000398 | GGCAGAGGAGGGCUGUUCUUCCC | 2.46 |
mmu‐miR‐381‐3p | MI0000798 | UAUACAAGGGCAAGCUCUCUGU | 2.42 |
mmu‐miR‐338‐3p | MI0000619 | UCCAGCAUCAGUGAUUUUGUUG | 2.40 |
mmu‐let‐7d‐3p | MI0000405 | CUAUACGACCUGCUGCCUUUCU | 2.38 |
mmu‐miR‐132‐3p | MI0000158 | UAACAUGCUACAGCCAUGGUCG | 2.22 |
mmu‐miR‐130a‐3p | MI0000156 | CAGUGCAAUGUUAAAAGGGCAU | 2.19 |
mmu‐miR‐744‐5p | MI0004124 | UGCGGGGCUAGGGCUAACAGCA | 2.17 |
mmu‐miR‐330‐5p | MI0000607 | UCUCUGGGCCUGUGUCUUAGGC | 2.13 |
mmu‐miR‐335‐3p | MI0000817 | UUUUUCAUUAUUGCUCCUGACC | 2.06 |
mmu‐miR‐29c‐3p | MI0000577 | UAGCACCAUUUGAAAUCGGUUA | 2.04 |
mmu‐miR‐181a‐1‐3p | MI0000697 | ACCAUCGACCGUUGAUUGUACC | 2.04 |
mmu‐miR‐872‐5p | MI0005549 | AAGGUUACUUGUUAGUUCAGG | 2.00 |
PCP vs Control 2.0‐fold change Downregulated miRNAs | |||
mmu‐miR‐143‐3p | MI0000257 | UGAGAUGAAGCACUGUAGCUC | 0.26 |
mmu‐miR‐15a‐5p | MI0000564 | UAGCAGCACAUAAUGGUUUGUG | 0.45 |
mmu‐miR‐1968‐5p | MI0009965 | UGCAGCUGUUAAGGAUGGUGGACU | 0.50 |
mmu‐miR‐582‐3p | MI0006127 | UAACCUGUUGAACAACUGAAC | 0.50 |
GFAP‐GFP transgenic mouse brains were rapidly removed 30 min after subcutaneous injection with PCP (4 mg/kg) or saline as control. GFP positive astrocytes were sorted by flow cytometry from prefrontal cortex. RNA was extracted from sorted astrocytes and used to prepare the miRNA sequencing libraries. The libraries were captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 36 cycles on Illumina HiSeq following the manufacturer's instructions. Trimmed reads were alignment to the miRBase pre‐miRNAs. miRNA read counts were normalized as tag counts per million miRNA alignments. Differentially expressed miRNAs (PCP vs Control >2.0 fold changes) were presented in the table.