Figure 5.

Ascorbic acid is unable to modulate lactate transport in cellular models of Huntington's disease. (A) Bar plot for ¹⁴C‐Lactate transport analysis using a 10‐s uptake assay (25°C) in Q7 and Q111 cells. Cells were treated with 0 mmol/L (control) or 1 mmol/L ascorbic acid prior the assay. The absolute values of ¹⁴C‐lactate uptake in controls were 553 (Q7 cells) and 554 (Q111 cells) pmol/min × 10⁶ cells. (B) Bar plots for the lactate uptake rate using BCECF H⁺ probe in Q7 and Q111 cells preloaded with 1 mmol/L ascorbic acid/0.1 mmol/L DTT (Asc) or 0.1 mmol/L DTT (control) for 60 min. The rate was obtained from the slope during the first 100 s after application of 5 mmol/L lactate. Data represent the mean ± SD from 42 cells (Q7) and 18 cells (Q111). ANOVA followed by Bonferroni posttest, ***P < 0.001. (C) and (D) Time course of lactate uptake rate using BCECF H⁺ probe in Q7 (C) and Q111 (D) cells preloaded with 1 mmol/L ascorbic acid/0.1 mmol/L DTT (Asc) or 0.1 mmol/L DTT (control) for 60 min. Data correspond to a representative experiment in Q7 (C) and Q111 (D) cells