Figure 6.

Specific knockdown of EP3 receptors ameliorated the cognitive function in mice after laparotomy. (A‐C) The open field test was comparable between the 2 group. Representative exploration traces are presented. (n = 10) (D‐E) There were no significant differences in pretone and tone freezing time between the 2 groups. Contextual freezing time was significantly increased by knocking down of EP3 expression. (n = 10) (F) The visualization of protein bands of pCREB, CREB, Arc, and BDNF. (G‐I) Quantitative analysis showed that hippocampal expression of pCREB, Arc, and BDNF in LV‐shEP3 injection mice was significantly higher than that in negative control lentivirus injection mice.(n = 3) Data presented as mean ± SEM. *P < 0.05, **P < 0.01 [Correction added on 03 April 2018, after first online publication: The word “GADPH” was changed to “GAPDH”; and “BNDF” was changed to “BDNF” in figure 6.]