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. 2017 Jan 11;28(3):880–893. doi: 10.1093/cercor/bhw410

Figure 2.

Figure 2.

Optogenetic inhibition of the bilateral caudal mPFC impaired the acquisition, recent and remote retrieval of DEC with the wCS, but not of DEC with the sCS. (A) Rats were injected with pAAV 2/9-CaMKIIα-eNpHR3.0-EYFP or pAAV 2/9-CaMKIIα-EYFP targeting the bilateral caudal mPFC. (B) Example of eNpHR3.0-EYFP expression in the bilateral caudal mPFC. White dashed line: optical fiber position. Scale bar, 500 μm. (C) Representative images showing cell-specific eNpHR3.0-EYFP expression (green) in pyramidal neurons (red) in mPFC. Scale bar, 20 μm. (D) Statistics of expression in pyramidal neurons (536 cells, from 5 rats). (E) Scheme for optical fiber implant site and 590-nm LED illumination pattern to bilateral caudal mPFC during each trial. (F–K) Top: Training and illumination phase protocol. Bottom: Effects of mPFC optogenetic inhibition during each trial on CR% of acquisition (F: n = 12 each; I: n = 12 eNpHR3.0: mPFC, n = 10 EYFP: mPFC), recent retrieval (G: n = 11 each; J: n = 12 eNpHR3.0: mPFC, n = 10 EYFP: mPFC), and remote retrieval (H: n = 11 eNpHR3.0: mPFC, n = 10 EYFP: mPFC; K: n = 11 each) of DEC with the sCS or wCS (N.S., not significant, **P < 0.01, ***P < 0.001; 2-way ANOVA with repeated measures followed by Tukey post hoc test or 2-tailed unpaired Student's t-test). Data are represented as mean ± s.e.m.