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. 2017 Jan 11;28(3):880–893. doi: 10.1093/cercor/bhw410

Figure 4.

Figure 4.

Optogenetic inhibition of the bilateral caudal mPFC axon terminals in the right PN abolished the acquisition, recent and remote retrieval of DEC with the wCS, but not of DEC with the sCS. (A) Rats were bilaterally injected with pAAV 2/9-CaMKIIα-eNpHR3.0-EYFP or pAAV 2/9-CaMKIIα-EYFP into the caudal mPFC. (B) Example of robust eNpHR3.0-EYFP expression in the axon terminals of mPFC in the PN. White dashed line: optical fiber position. Scale bar, 500 μm. (C) Representative images showing images of eNpHR3.0-EYFP-positive axons (green) innervating target neurons in the PN (NeuN, red). Scale bar, 20 μm. (D) Coronal schematic of optical fiber implant site and 590-nm LED illumination pattern to the caudal mPFC axon terminals in the PN during each trial. (E–J) Top: Training and illumination phase protocol. Bottom: Effects of optogenetic inhibition of mPFC axon terminals in the PN during each trial on CR% of acquisition (E: n = 12 eNpHR3.0: mPFC–PN, n = 10 EYFP: mPFC–PN; H: n = 10 eNpHR3.0: mPFC–PN, n = 11 EYFP: mPFC–PN), recent retrieval (F: n = 12 eNpHR3.0: mPFC–PN, n = 11 EYFP: mPFC–PN; I: n = 9 eNpHR3.0: mPFC–PN, n = 10 EYFP: mPFC–PN), and remote retrieval (G: n = 11 eNpHR3.0: mPFC–PN, n = 9 EYFP: mPFC–PN; J: n = 10 eNpHR3.0: mPFC–PN, n = 11 EYFP: mPFC–PN) of DEC with the sCS or wCS (N.S., not significant, **P < 0.01, ***P < 0.001; 2-way ANOVA with repeated measures followed by Tukey post hoc test or 2-tailed unpaired Student's t-test). Data are represented as mean ± s.e.m.