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SDS-PAGE analysis of purification of recombinant (a) loop and (b) cork domain. (a) Lane 1: expression of recombinant loop domain, Lane F: unbound protein flow, Lanes W1–W3: column washed with buffer, Lane E1: column washed with elution buffer. (b) Lane 1: expression of recombinant cork domain, Lane F: unbound protein flow, Lanes W1–W3: column washed with buffer, Lanes E1–E3: column washed with elution buffer.
