Table 2.

Box–Behnken experimental design of 3 factors and 3 levels

Run	A (°C)	B (rpm)	C (pH)	Protein expression (μg/ml)	Biomass production, dry weight (mg/ml)
1	30	300	7.5	60.1	1.58
2	30	300	7.5	65.3	1.63
3	23	300	6.5	31.3	1.46
4	30	200	6.5	44.4	1.24
5	23	300	8.5	94.5	1.6
6	37	200	7.5	74.5	1.32
7	30	400	8.5	143.2	1.5
8	37	400	7.5	60	1.7
9	30	300	7.5	69.2	1.62
10	30	400	6.5	91.4	1.8
11	37	300	8.5	140.7	1.42
12	23	200	7.5	56.2	1.26
13	23	400	7.5	43.7	2.04
14	30	200	8.5	185.2	1.42
15	37	300	6.5	37.2	1.6