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1H NMR spectrum of PEGDE crosslinked hydrogel disaccharide unit after enzymatic digestion (sample #1). Signals due to the protons in the repetitive unit –C2H4O– in PEG (red) and to the methyl group on the HA chain (blue) are evidenced. The signals from the remaining 10 PEG protons overlap the HA ones (3.2-4.0 ppm) completely
