Table I.
Distribution of marker compounds and marker enzyme activities in subcellular fractions of rosemary leaves
Marker | Chloroplast | Endoplasmic Reticulum | Golgi Apparatus | Plasma Membrane |
---|---|---|---|---|
Chlorophylla | 185.2 ± 1.0 (100) | 0 (0) | 0 (0) | 0 (0) |
NADPH-Cyt c reductaseb | ND c (0) | 38.9 ± 0.7 (100) | 11.5 ± 1.0 (30) | 7.5 ± 2.8 (19) |
Latent IDPased | 1.8 ± 0.4 (<1) | ND (0) | 153.9 ± 5.7 (100) | 33.8 ± 5.6 (22) |
Vanadate-sensitive ATPased | ND (0) | ND (0) | 1.4 (<1) | 295.8 ± 60.6 (100) |
Cyt c oxidased | 2.6 ± 0.65 | 2.2 ± 0.2 | 0.7 ± 0.5 | 0.8 ± 0.1 |
Data are shown in absolute values and as a percentage of the maximum for each marker (in brackets). Results correspond to the mean ± se of triplicate experiments.
mg (chlorophyll a + b) g−1 protein.
nmol Cyt c min−1 mg−1 protein.
ND, Not detected.
μmol phosphate min−1 mg−1 protein.