Fig 3. Cuticle permeability defects in ale1-4 and gso1-1 gso2-1 seedlings do not correlate with changes in cutin load.

(a) Cotyledons grown in vitro for 5 days under continuous light were collected, delipidated and their cutin content and composition was analyzed as described in the Material and Method section. ωOH and DCA stand respectively for omega-hydroxy acid and α,ω-dicarboxylic acid. Mean values are shown in μg/mg of delipidated dry residue (DR) ± SD of three replicates. Statistical differences were determined according to a Student’s t test: *** denotes p<0.0001, ** denotes p<0.001 and * denotes p<0.01. (b) Cuticle permeability to toluidine blue in etiolated seedlings from the genotypes tested in (a).