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(A) SRS frame at 2930 cm−1 of two fixed cells using unamplified Stokes pulse. (B) SRS spectra at four individual pixels A, B, C, D and E with neglible water signal in the area surrounding the fixed cells. (C) SRS frame at 2930 cm−1 with amplified Stokes pulse by pumping the amplifier at 2.5 W. (D) SRS spectra of four individual pixels A, B, C, D, and E. Images are acquired at 8 μs pixel dwell time. IP and IS = 40 mW.
