Fig. 8.
Live whole-cell 3D imaging using socSPIM. a: Maximum intensity projections in xy and yz at t = 10 s of lysosomes in HEK cells labelled with mEos4b-LAMP1. b: Same as (a) with Richardson-Lucy deconvolution. c: 3D volume rendering of deconvolved lysosome data, color-coded by depth in μm. d: A large complex diffuses slowly until it suddenly splits into two complexes as indicated by blue circles. e: An initially slowly diffusing lysosome can be seen to suddenly undergo fast, directed motion (red line shows the local 10 tracks backward). The scale bar in a-b is 5 μm and the scale bar in d-e is 1 μm.