Figure 1. Experimental design and phylogenies.

(A) Four species of Drosophila were independently infected both with a sigma virus with which they are naturally infected with in nature (red) and two viruses that naturally infect another species (black). (B) Phylogenies of the sigma viruses (inferred using the L gene) and their Drosophila hosts (inferred using COI, COII, 28S rDNA, Adh, SOD, Amyrel and RpL32 genes), redrawn from Longdon et al. (2015a) and Longdon et al. (2015b). Scale bars represent substitutions per site under a relaxed clock model. Posterior supports for nodes are all >0.99.