Table 1. Statistical parameters for two-way ANOVA.
| Parameter | Figure | Factor 1 | Factor 2 | Interaction |
|---|---|---|---|---|
| Firing frequency | Figure 1b | steroid F (2, 57)=14.7 * |
genotype F (1, 57)=40.1 * |
F (2, 57)=6.2 * |
| Input-output curve |
Figure 1f intact OVX OVX + E |
current F (4, 80)=242.7 * F (4, 72)=138.6 * F (4, 84)=182.2 * |
genotype F (1, 20)=8.2 * F (1, 18)=0.8 F (1, 21)=6.6 * |
F (4, 80)=0.6 F (4, 72)=0.7 F (4, 84)=1.5 |
| IT current density | Figure 2b | voltage F (8, 104)=39.74 * |
genotype F (1, 13)=11.1 * |
F (8, 104)=9.4 * |
| IT normalized conductance | Figure 2c activation inactivation | voltage F (8, 104)=494.7 * F (8, 104)=195.8 * |
genotype F (1, 13)=3.2 F (1, 13)=4.5 * |
F (8, 104)=1.5 F (8, 104)=3.1 * |
| LH |
Figure 3f
Figure 3g |
AAV type F (1, 12)=29.8 * F (1, 13)=0.3 |
time F (2, 24)=2.1 F (1, 13)=35.8 * |
F (2, 24)=1.8 F (1, 13)=19.5 * |
| Input-output curve |
Figure 4d
IF post hoc PCR post hoc AAV-Esr1 vs KERKO |
current F (4, 136)=165.5 * F (4, 68)=123 * F (4, 100)=154.7 * |
AAV type F (2, 34)=7.2 * F (1, 17)=12.5 * F (1, 25)=2.1 |
F (8, 136)=0.7 F (4, 68)=4.3* F (4, 100)=7.2 |
| Days proestrus/week | Figure 5d | time F (1, 12)=13.6 * |
AAV type F (2, 12)=5.8 * |
F (2, 12)=10.0 * |
| LH |
Figure 5g kisspeptin GnRH |
injection F (1, 12)=34.8 * F (1, 12)=20.0 * |
AAV type F (1, 12)=4.7 # F (1, 12)=7.0 * |
F (1, 12)=17.1 * F (1, 12)=7.5 * |
| steroids | genotype | interaction | ||
| Input resistance | Figure 1—figure supplement 1a | F (2, 59)=2.6 | F (1, 59)=13.2 * | F (2, 59)=2.0 |
| Cell capacitance | Figure 1—figure supplement 1b | F (2, 59)=5.2 | F (1, 59)=0.1 * | F (2, 59)=0.4 |
| Normalized uterine mass | Figure 1—figure supplement 1c | F (2, 30)=19.9 * | F (1, 30)=80.0 * | F (2, 30)=2.4 |
*p<0.05, # p=0.05.