Table 4. Primer probes used for single-cell qPCR.
| IDT prime time qPCR probe assay |
Transcript | Forward 5'−3' | Reverse 5'−3' | Probe 5'−3' | Amplicon (bp) |
Accession no. |
Location |
|---|---|---|---|---|---|---|---|
| Mm.PT.58.42702897 | Cacna1g | CTCAACTGTATCACCATCGCTA | AAGACTGCCGTGAAGATGT | CGCCCCAAAATTGACCCCCAC | 101 | NM_009783 | 4446–4546 |
| Mm.PT.58.15908160 | Cacna1h | GACACTGTGGTTCAAGCTCT | TTATCCTCGC TGCATTCTAGC |
ACCTTGGTCTTCTTTTCATGCTCCTGT | 122 | NM_021415 | 5565–5686 |
| Mm.PT.58.9567566 | Cacna1i | CATCACCTTCATCATCTGCCT | CCTCCAGCACAAAGACAGT | ACCAGCCTACATCCCTAGAGACAGC | 125 | NM_001044308 | 4914–5038 |
| Mm.PT.58.41764708 | Esr1 | GCTCCTTCTCATTCTTTCCCA | TCCAGGAGCAGGTCATAGAG | CCATGCCTTTGTTACTCATGTGCCG | 108 | NM_007956 | 1768–1865 |
| Mm.PT.58.16981577 | Esr2 | CCTCCTGATGCTTCTTTCTCAT | TCGAAGCGTGTGAGCATTC | TCCATGCCCTTGTTACTGATGTGCC | 133 | NM_207707 | 1829–1961 |
| Mm.PT.58.30501833 | Hcn1 | GCGTTATCACCAAGTCCAGTA | CAGTAGGTATCAGCTCGGACA | CTCCGAAGTAAGAGCCATCTGTCAGC | 115 | NM_010408 | 1913–2027 |
| Mm.PT.58.7963736 | Hcn2 | CTTCACCAAGATCCTCAGTCTG | GGTCGTAGGTCATGTGGAAA | TGCGGCTATCACGGCTCATCC | 98 | NM_008226 | 935–1032 |
| Mm.PT.58.7999585 | Hcn3 | GCCTCACTGATGGATCCTACT | TCAAGCACCGCATTGAAGT | ACCTATTGTCGCCTCTACTCGCTCA | 130 | NM_008227 | 1546–1675 |
| Mm.PT.58.43863085 | Hcn4 | GCTGATGGCTCCTATTTTGGA | TCATTGAAGTTGTCCACGCT | AAGTATCCGCTCTGACGCTGGC | 116 | NM_001081192 | 2614–2729 |
| Mm.PT.45.16269514 | Kiss1 | CTGCTTCTCCTCTGTGTCG | TTCCCAGGCATTAACGAGTTC. | CGGACTGCTGGCCTGTGGAT | 105 | NM_178260 | 66–170 |
| Mm.PT.47.10254276 | Pgr | CGCCATACCTTAACTACCTGAG | CCATAGTGACAGCCAGATGC | AGATTCAGAAGCCAGCCAGAGCC | 124 | NM_008829 | 2230–2353 |
| Mm.PT.51.17048009.g | Syn1 | CTTGAGCAGATT GCCATGTC | ACCTCAATAATGTGATCCCTTCC | ACGTGTCTACCCACAACTTGTACCTG | 131 | NM_013680 | 1159–1289 |
| Mm.PT.58.33106186 | Th | CCCTACCAAGATCAAACCTACC | CTGGATACGAGAGGCATAGTTC | TGAAGCTCTCTGACACGAAGTACACCG | 96 | NM_009377 | 1298–1393 |