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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Bone. 2019 Mar 19;123:92–102. doi: 10.1016/j.bone.2019.03.020

Fig. 5. PTEN is a target of miR-181a/b in DDCs.

Fig. 5.

A conserved binding site in the 3’UTR of PTEN is shown to interact with the seed sequence (grey shaded area) of the mature 5p strand of human (hsa) miR-181a or miR-181b (A). Representative Western blot image showing decreased protein levels of PTEN in DDCs following 24 h transduction with LV-181a/b-1 compared to LV-NS (B). Quantification of PTEN protein expression relative to β-actin (C) or PTEN mRNA expression relative to PPIA (D). Data in (C) and (D) are expressed ± SD; n = 4 (C); n = 3 (D). ****p < 0.0001.