Fig. 2.

Distinct features of NEnCs and TEnC lumens. A schematic indicating the differences expected between normal and tumor vessels is depicted in a) and b), respectively. In vitro NEnC c) and TEnC d) phalloidin-stained vessels. e) Lumen confluency for NEnC and TEnC lumens (**p = .001) calculated with F-actin images. f) Anisotropy index calculated with Fibriltool ImageJ macro (*p = ∙0489). Histogram of the angle of orientation for F-actin fibers in NEnC lumens g) and in TEnC lumens h). Calculations were performed using 90° is the axis parallel to the lumen. Comparisons in e), f) and between g-h) were performed with Student's t-test with Welch correction after passing the Shapiro-Wilk normality test. Distributions were statistically different **p = ∙0091 via the Kolmogorov-Smirnov test, even though the central tendency was not found to be significantly different between distributions (p = ∙4460). Graphs represent mean ± SEM. At least 8 lumens and 2 different patients were used in these experiments.