Fig. 3.

Secretory profile of NEnC and TEnC lumens. a) Schematic indicating the seeding procedure and media collection for cytokine analysis. In vitro vessel secretion for inflammatory and angiogenic cytokines (G-CSF, FGF-1, FGF-2, VEGF-A, VEGF-C, VEGF-D, and Endothelin) in normal and tumor endothelial cells are depicted in b) and c) for patient 22 and patient 28 respectively, illustrating inter-patient heterogeneity. Comparisons were performed between NenC and TenC via multiple Student's t-tests with false discovery rate method of Benjamini, Krieger and Yekuteli. (n = 3, pooled samples over two days from at least six lumens) d) qPCR characterization of differences between NEnC and TEnC lumens. Expression fold changes of angiogenesis-related markers were calculated for primary NEnC and TEnC lumens from patient 51 and results of TEnC lumens were normalized to NEnC lumens. Graphs represent mean ± SEM. (n = 3, pooled samples from at least two lumens.)