Fig. 4.

Drug response of NEnC and TEnC lumens. Representative bright field images of TEnCs vessels for patient 22 treated with vehicle (DMSO) or an antiangiogenic agent. Results are shown for 1 μM of Pazopanib a) and 1 μM of Sunitinib at 0 and 48 h after drug treatment, as number of sprouts present in the treated lumens, minus the sprouts of the vehicle control. Responses of NEnC c) and TEnC d) lumens, quantified as the number of sprouts per lumen minus the sprouts of the vehicle control are shown for five different patients (22, 31, 38, 39 and 41), both to Sunitinib and Pazopanib. Dose-response curves of TEnC lumens to Pazopanib of lumens from patients 31 and 38 e-f) and Sunitinib g-h) for patients 22 and 38, illustrating inter-patient heterogeneity. Graphs represent mean ± SEM. At least three lumens were used with three independent experiments performed per condition.