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. 2019 Apr 30;9:6663. doi: 10.1038/s41598-019-43248-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Real-time qPCR analysis for the validation of gene expression of the following epigenetic modulators: (a) Ezh2, (b) DNMT3a, (c) DNMT1, (d) RunX3 and (e) LSD1. The mice were treated with corn oil or PCN (50 mg/kg/day) for 3 days followed by saline or LPS (2 mg/kg) for 16 h. A few select candidate genes from the list of epigenetic factors that were differentially regulated between the PCN and LPS treatments were chosen for the validation of gene expression. *p < 0.05 compared to the control treatment. ^#p < 0.05 compared to PCN or LPS treatment alone.