Table 2.
SPR analysis on the binding of selection products with the target protein used for the selection (TH1N1).
| Ligand/Target | kon (M−1s−1) | koff (M−1s−1) | KD (M) | Rmax (RU) |
|---|---|---|---|---|
| Random ligand pool/TH1N1 | not sufficiently bound | 16 | ||
| SELEX pool for TH1N1/TH1N1 | 6.30×103 | 1.89×10−4 | 2.99×10−8 | 809 |
| Compe-SELEX pool for TH1N1/TH1N1 | 9.34×103 | 9.41×10−7 | 1.01×10−10 | 2651 |
| Compe-SELEX pool for TH3N2/TH1N1 | 9.06×103 | 1.80×10−3 | 1.99×10−7 | 321 |
| TH1N1-Apta03/TH1N1 | 3.74×104 | 3.08×10−6 | 0.82×10−10 | 603 |
| Monoclonal antibody/TH1N1 | 2.33×105 | 5.90×10−8 | 0.25×10−12 | 694 |
| RHA0006/TH1 (Ref.*) | NA | NA | 1.53×10−8 | NA |
‘Pool’ indicates a set of DNA aptamers that were just selected. A single cycle kinetics analysis was adopted for the SPR (surface plasmon resonance).