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. 2019 Apr 30;10:1993. doi: 10.1038/s41467-019-10000-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Ion pump activity of KR2 mutants. Ion transport activities of the KR2 wildtype (WT) and mutants were assayed by monitoring the pH changes in the external media of the suspension of E. coli cells (a) and the relative initial slopes of the pH change of KR2 WT, P219T, S254A, and P219T/S254A (b). The initial slopes of Na⁺ and H⁺ pumps were determined from the results of NaCl + CCCP and CsCl, respectively, shown in a. The light was illuminated at t = 0–150 s. Source data are provided as a Source Data file