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. 2019 Apr 30;10:1993. doi: 10.1038/s41467-019-10000-x

Fig. 4.

Fig. 4

Light-induced infrared absorption changes of the KR2 mutants. Light-induced Fourier transform infrared difference spectra of a KR2 wildtype (black) and the mutants (red), b P219T, c S254A and d P219T/S254A, in the 1580–1480 (left), 1225–1155 (middle) and 1050–880 cm−1 (right) regions at T = 77 K and pH 8.0. Solid and dotted lines represent the samples hydrated with H2O and D2O, respectively. Source data are provided as a Source Data file