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. 2019 Apr 24;9:122. doi: 10.3389/fcimb.2019.00122

Figure 3.

Figure 3

Analysis of the expression pattern of Ts-FBPA at different developmental stages of T. spiralis. (A) Revervse transcription qPCR analysis of the expression of Ts-FBPA at different developmental stages of T. spiralis. After reverse transcription, the cDNA of ML, AD3, AD6, and NBL was used as a template for qPCR using SYBR Green. Each reaction was performed in triplicate. All fold changes were relative to the NBL stage. Significant differences are as follows: ***P < 0.001. (B) Western blot analyses of Ts-FBPA expression levels at different developmental stages of T. spiralis. Expression levels of Ts-FBPA protein (41 kDa) in a crude antigen preparation of diverse T. spiralis growth phases were determined by Western blotting with 1:500 dilutions of anti-rTs-FBPA serum. Line ML: T. spiralis ML crude parasite antigen, Line AD3: T. spiralis AD3 crude parasite antigen, Line AD6: T. spiralis AD6 crude parasite antigen, Line NBL: T. spiralis-NBL crude parasite antigen, Line ES: T. spiralis-ML ESPs.