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. 2019 Apr 30;9:6677. doi: 10.1038/s41598-019-43060-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of virulence phenotypes of P. aeruginosa strains isolated from cystic fibrosis patients. P. aeruginosa CF patient isolates were compared to laboratory strain PA19660 Xen5. All assays were conducted at 37 °C for 24 h. (A) Colony diameter of swimming, or flagellar, motility assay conducted on soft (0.3%) agar. (B) Twitching, or pili, motility assay conducted on semi-hard (1.3%) agar. Colony diameter was measured across point of inoculation to the edges of bacteria colony. (C) Biofilm formation assay was conducted in 96-well microtiter plates. Biofilms were stained with crystal violet (0.1%), solubilized in ethanol (95%), and absorbance measured at OD₅₅₀. Bars are the mean ± SD; n = 5 to 6 replicates in motility assays and n = 10 in biofilm assay. Each assay was performed at least three separate times and representative results are shown.