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. 2019 Apr 30;9:6711. doi: 10.1038/s41598-019-43160-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Organ and experiment had a significant effect on the tissue spirochete load of B. afzelii in the bank voles. The spirochete loads were standardized per mg of DNA and then log10-transformed. For the Finnish bank voles, the tissue spirochete loads were higher compared to the Swiss bank voles. The rank order of the organ spirochete loads (from lowest to highest) depended on the experiment: bladder, ear, ventral skin, ankle joint for the Swiss bank voles and bladder, ventral skin, ankle joint, ear for the Finnish bank voles. Shown are the medians (black line), the 25th and 75th percentiles (edges of the box), the minimum and maximum values (whiskers), and the outliers (circles).