Table 3.
Effect of a short-term feeding (4 days) of a FFC or control diet ± metformin on caloric intake, body weight gain, markers of liver damage.
| groups | P-value | ||||||
|---|---|---|---|---|---|---|---|
| C | FFC | C + Met | FFC + Met | DExME | ME | DE | |
| Caloric intake [kcal/mouse/d] | 10.3 ± 0.4 | 10.6 ± 0.3 | 10.1 ± 0.2 | 10.3 ± 0.6 | NS | NS | NS |
| Body weight [g] (at end of trial) | 19.0 ± 0.5 | 20.0 ± 0.4 | 20.1 ± 0.4 | 19.9 ± 0.5 | NS | NS | NS |
| Weight gain [g] | 1.0 ± 0.5 | 1.7 ± 0.2 | 1.6 ± 0.3 | 1.3 ± 0.8 | NS | NS | NS |
| Liver weight [g] | 1.0 ± 0.1 | 1.2 ± 0.1 | 1.0 ± 0.0 | 1.2 ± 0.1 | NS | NS | <0.05 |
| Liver:Body weight ratio [%] | 5.4 ± 0.3 | 6.1 ± 0.2c | 5.2 ± 0.1 | 6.1 ± 0.3c | NS | NS | <0.05 |
| Hepatic triglycerides [µg/mg protein] | 13.1 ± 2.5 | 54.3 ± 5.6a,c,d | 17.9 ± 2.8 | 31.5 ± 3.9a | <0.05 | <0,05 | <0.05 |
| Plasma ALT [U/I] | 17.1 ± 2.1 | 18.2 ± 1.8 | 14.7 ± 1.5 | 13.0 ± 1.8 | NS | <0.05 | NS |
| F4/80 positive cells (number/microsc. field) | 20.6 ± 1.1 | 25.0 ± 0.7a,c | 20.9 ± 0.8 | 21.5 ± 1.2 | NS | NS | <0.05 |
Values are means ± standard error of means; n = 7–8. ALT: alanine aminotransferase, C: control diet, C + Met: control diet and oral treatment with metformin (300 mg/kg BW/day), DE: diet effect, DExME: interaction between diet and metformin, FFC: fat-, fructose- and cholesterol-rich diet, FFC + Met: fat-, fructose- and cholesterol-rich diet and oral treatment with metformin (300 mg/kg BW/day), ME: metformin effect, Met: metformin, NS: not significant. aP < 0.05 compared with mice fed a control diet; cP < 0.05 compared with mice fed a control diet treated with metformin;. dP < 0.05 compared with mice fed a FFC diet treated with metformin.