Fig. 4.

The TaPin1-PKM2 axis is important for glucose uptake and transformation. a Glucose uptake (fluorescence intensity) decreased in Theileria-infected TBL3 cells upon TaPin1 inhibition by Buparvaquone or Juglone drugs. b, c Ectopic bovine PKM2 partially rescued glucose uptake following TaPin1 inhibition with Buparvaquone (b) or Juglone (c) in TBL3 cells. d PKM2 knockdown reduced glucose uptake in parasitized TBL3 cells (Fluorescence intensity). e Inhibition of TaPin1 by Buparvaquone or Juglone affected TBL3 colony formation in soft-agar. f siPKM2 reduced TBL3 cell colony formation in soft agar. Data represent three independent experiments (average ± sd). The p-values were calculated using the Bonferroni test for multiple comparisons (a–e) and the Mann–Whitney test (f). *p < 0.05, **p < 0.01, ***p < 0.001