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. 2019 Apr 24;10:781. doi: 10.3389/fimmu.2019.00781

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Copyright © 2019 Wan, Zhang, Zhao, Cheng, Gao, Wang, Xu, Zou and Gu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of PcrV_NH-elicited immune responses. (A) PcrV_NH-elicited humoral immune response. The bar represents the titer of IgG against PcrV_NH, MBP-N_ter, MBP-H₁₂, and MBP-PcrV_{full−length} from PcrV_NH-immunized mice (B). The subtypes of anti-PcrV_NH antibodies. The bar represents the titer of IgG1, IgG2a, and IgG2b against PcrV_NH 7 days after the last immunization. (C) PcrV_NH-elicited cellular immune response in the spleen. Splenocytes from PcrV_NH-immunized mice were collected and stimulated with purified antigens. The bar represents the concentration of IL-4, IFN-γ, and IL-17A in the culture supernatant. The data are shown as the mean ± SE. Multiple comparisons among three groups were analyzed by one-way ANOVA. *P < 0.05, ns, no significance (P > 0.05).