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. 2019 Apr 24;13:329. doi: 10.3389/fnins.2019.00329

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Copyright © 2019 Gaudioso, Garcia-Rozas, Casarejos, Pastor and Rodriguez-Navarro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lipidomic composition of brain’s mice mitochondria change due to aging and parkin loss. Lipidomic analysis and enrichment of mitochondrial fraction isolated from WT and PK-KO mice’s brain. (A) Heatmap of lipidomic classes detected and changes due to aging and parkin loss expressing the relative differences between the groups after normalization and the hierarchical clustering. (B) 2D representation of the Partial Least Square Discriminant Analysis of brain’s mitochondrial lipidomic composition. (C) Western-Blot experiments showing homogenates and mitochondria stained for Cytochrome C, VDAC, and TOM20. (D) Quantification of Cyt C signal intensity in homogenates and mitochondria. (E) Quantification of VDAC signal intensity in homogenates and mitochondria. (F) Quantification of TOM20 signal intensity in homogenates and mitochondria. n = 4–6 for western blot analysis. Graphs represent the mean ± SEM. Panels A,B were made using MetaboAnalyst software. Statistical analysis of western blot experiments was done by One Way ANOVA test. OH Cer, hydroxylated ceramide; OH HexCer, hydroxylated hexosylceramide; HexCer, hexosylceramide; SM, sphingomyelin; CL, cardiolipin; PE, phosphatidylethanolamine; LPE, lysophosphatidylethanolamine; PG, phosphatidylglycerol; dhCer, dihydroceramide; PC, phosphatidylcholine; Cer, ceramide; PI, phosphatiylinositol; PS, phosphatidylserine; LPC, lysphosphatidylcholine; Hom, homogenate; Mito, mitochondria; Cyt C, cytochrome C; Y, young; O, old.