Skip to main content
. 2019 Apr 24;10:882. doi: 10.3389/fimmu.2019.00882

Figure 1.

Figure 1

Foxp3-targeted Nrp1 deletion only occurs on Treg cells and does not affect conventional T cell phenotype. Peripheral lymph nodes (pLN), spleen (Spl), and blood were harvested from Foxp3YFP/CreNrp1+/+ (wt, dark green bars), Foxp3YFP/CreNrp1+/flox (het, light green bars), and Foxp3YFP/CreNrp1flox/flox (Nrp1KO, white bars) animals. Leukocytes were obtained and tested for the indicated markers by flow cytometry. (A) Graphs show the frequency of Nrp1, Eos, CD49b, and CD25 on Foxp3+ Treg cells and conventional CD4+T (convT) cells from wt, het, and Nrp1KO Treg cell-restricted mice. (B) Representative histograms showing the expression of indicated markers on Foxp3+ Treg cells from wt (dark green line), het (light green line), Nrp1KO (black line) mice; and FMO control (gray line). (C) Immunoblot of Nrp1 protein expression on wt and Nrp1KO Treg cells. For A, n = 3–4 animals per genotype, each circle represents one mouse, bars represent mean. Two-way ANOVA, * < 0.05; ** < 0.01; *** < 0.001; ns, not significant. Representative data of three independent experiments.