Figure 4.

Nrp1KO Treg cells are unable to exert long-term skin transplantation tolerance. Conventional CD4+ T cells were sorted and adoptively transferred alone or with sorted Foxp3/YFP+CD45.2+ Treg cells into RAG-KO recipient mice. Next day, mice were transplanted with F1 semi-allogeneic skin grafts (day 0) and graft survival was monitored over time. Long-term skin allograft survival was compared between mice receiving wt (dark green), het (light green), or Nrp1KO (black) Treg cells. Lines represent graft survival (%), log-Rank test: ^*p < 0.05 between “convT cells” and “wt”; ^*p < 0.05 between “convT cells” and “het”; ^*p > 0.05 between “convT cells” and “Nrp1KO”; ^*p > 0.05 between “wt” and “het”; ^*p < 0.05 between “wt”and “Nrp1KO”.