Figure 5.

Nrp1+Treg cells are required for the acquisition of Nrp1 and Eos expression by conventional CD4+ T cells during skin transplantation acceptance. Mice were transplanted as described in Figure 4. At day 20 post-surgery, graft-draining lymph nodes were harvested and cells were analyzed for Nrp1 and Eos expression by flow cytometry. Representative contour plots depicting (A,B) Nrp1 and (C,D) Eos expression on gated live CD4+CD45.1+ convT cells. (B–D), bars represent mean and each circle represents one mouse. Unpaired T-test, ^* < 0.05; ^** < 0.01; ns, not significant; PS, post sorting. Representative data of at least two independent experiments.