Figure 2.

Healthy primary keratinocytes (A) and fibroblasts (B) were treated with Apt-21-2 Cy3 (100 nM; red) or FITC-conjugated poly(I:C) (1 μg/ml; green) in the presence or absence of LL-37 (2.5 μM) for 24 h. Cells were washed with acid to remove extracellular RNA and imaged by confocal microscopy to assess uptake of Apt 21-2 and poly(I:C). Nuclei were visualized with 4′-6-diamidion-2-phenylidole (DAPI) (bars = 20 μm). Images are representative of three independent experiments.