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. 2019 Apr 24;10:421. doi: 10.3389/fphar.2019.00421

FIGURE 4.

FIGURE 4

The endothelial protective action of RES against HG is SIRT1 dependent, in vivo. (A) The presence of immunofluorescence with CD31 and Ki67 of wounded skin tissue sections, scale bars = 30 μm (×400), (C) enlargement of the indicated area in (A), scale bars = 12 μm (×1000), (D) confocal immunofluorescence with CD31 and c-Caspase-3 of wounded skin tissue sections, scale bars = 30 μm (×400), (F) enlargement of the indicated area in (D), scale bars = 12 μm (×1000), and (G) images of skin wounds, from db/m mice, db/db mice and db/db mice receiving RES (10 μM) or vehicle treatment with saline smeared on the wound, n = 6 mice in each group. For signaling pathway analysis, EX-527 (10 μM) was injected intradermally into the wound edges in the mice after RES smeared on the wound. Quantification of the proportion of Ki67 positive staining of endothelial cells (B), c-Caspase-3 positive staining of endothelial cells (E), and wound areas (H). All values displayed are means ± SEM of 8 independent experiments. #p < 0.05 vs. db/m mice; ∗p < 0.05 vs. db/db mice or vehicle treated db/db mice; % p < 0.05 vs. db/db mice receiving RES.