Figure 2.

The inhibition of glycolysis after maturation significantly impairs the function of mature CD137L-DCs. DCs were generated and on day 8 treated with 50 mM 2-DG for 24 h. (A) Cell surface expression of co-stimulatory and inhibitory molecules was measured by flow cytometry. (B) The secretion of cytokines by DCs was measured by ELISA. Depicted are the means ± standard deviations of relative changes upon addition of 2-DG to respective medium controls (values set at 1) from up to six independent experiments with DCs from different donors. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 (two-tailed, one sample student t test). MFI, geometric mean fluorescence intensity. n.d., not detected.