Skip to main content
. 2019 Apr 24;9:308. doi: 10.3389/fonc.2019.00308

Figure 1.

Figure 1

Functional assays showing migration of control transfected (Ct) and HBx transfected (HBx) Huh7 cells. (A) Phase contrast images of transwell assays showing migration of Ct and HBx cells from the upper chamber toward lower chamber containing media, LX2 cells or HUVECs after 24 h (magnification at 4X). (B) Bar diagram showing the number of migrated Ct and HBx cells in transwell assays under different conditions. (C) Phase contrast images showing migration of Ct and HBx cells after creation of a scratch/wound in presence of media alone, conditioned media (CM) from HUVECs (HUVECs-CM) or CM from LX2 cells (LX2-CM) at 0 and 24 h (magnification at 10X). (D) Bar diagram showing average relative wound width of Huh7 cells under different conditions. The relative wound width (μm) were calculated as wound width at time point 24 h divided by wound width at time point 0 h. Data is represented as mean ± SD (n = 3 each). *p < 0.05.