Table 4.
Effect of dietary electrolyte balance (DEB), dissolved oxygen levels and acute stress on energy use in liver of rainbow trout.
| DEB 200 |
DEB 700 |
Factors |
Interactions |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Normoxia |
Hypoxia |
Normoxia |
Hypoxia |
||||||||||||
| C | S | C | S | C | S | C | S | D | O | S | DOS | DO | DS | OS | |
| LDH | 2699.12 ± 274.64 | 3349.83 ± 437.29 | 2990.73 ± 298.11 | 3092.40 ± 315.63 | 2287.73 ± 281.52 | 2579.46 ± 204.78 | 2754.66 ± 114.60 | 2606.28 ± 297.18 | ∗ | ns | ns | ns | ns | ns | ns |
| IDH | 84.64 ± 4.95 | 85.89 ± 8.59 | 70.13 ± 4.98 | 74.58 ± 4.99 | 94.09 ± 3.82 | 93.19 ± 4.51 | 80.05 ± 3.12 | 76.34 ± 4.20 | ns | ∗∗ | ns | ns | ns | ns | ns |
| LDH/IDH | 32.43 ± 3.48 | 39.30 ± 3.26 | 43.01 ± 3.18 | 42.69 ± 4.69 | 25.08 ± 3.64 | 28.20 ± 2.58 | 34.83 ± 1.96 | 33.56 ± 2.89 | ∗∗ | ∗ | ns | ns | ns | ns | ns |
| Ec | 211.37 ± 23.23 | 175.51 ± 12.78 | 268.48 ± 28.93 | 182.61 ± 22.62 | 183.38 ± 13.03 | 205.08 ± 15.22 | 199.70 ± 18.66 | 177.93 ± 22.04 | ns | ns | ns | ns | ns | ∗ | ns |
Lactate dehydrogenase (LDH) and iso-citrate dehydrogenase (IDH) activities are expressed as nmol min-1 mg-1 tissue. Energy consumption value (Ec) is expressed as mJ mg-1 weight wet tissue. Values are mean of 9 fish±SEM. C, Control; S, Acute stress. Three-way ANOVA analysis results; D, Diet; O, dissolved oxygen levels; DOS, DO, DS, and OS, Interactions; ns, not significant P > 0.1; ∗P < 0.05; ∗∗P < 0.01.