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. 2019 Apr 24;6:45. doi: 10.3389/fcvm.2019.00045

Figure 2.

Figure 2

Validation of Akita/miR-133aTg mice. (A) Genotyping for WT, Akita, Akita/miR-133aTg, and miR-133aTg mice with Insulin 2 and miR-133aTg primers. There were two bands in Akita (Insulin 2 heterozygous) at 140 and 280 bp. There was one band for the miR-133aTg at 670 bp. (B) Blood glucose levels for WT, Akita, Akita/miR-133aTg, and miR-133aTg mice. There was no significant change in the blood glucose levels of Akita and Akita/miR-133aTg. (C) Gene expression levels of miR-133a isolated from the left ventricle of WT, Akita, Akita/miR-133aTg, and miR-133aTg hearts. (D) miR-1 quantification by individual miR-1 assay. Values are presented as mean ± SEM. One-way analysis of variance (ANOVA) followed by Tukey's test was used for statistical significance. N = 4–5 per group. *P < 0.05; ***P < 0.001.