Figure 4.
Cardiac-specific miR-133a prevents DM-induced cardiac fibrosis. (A) Picrosirius red staining of the heart from WT, Akita, Akita/miR-133aTg, and miR-133aTg mice. Values are presented as mean ± SEM. One-way analysis of variance (ANOVA) followed by Tukey's test was used for statistical significance. N = 4 per group. *P < 0.05. (B) In situ zymography for collagenase activity was performed on the heart sections of WT, Akita, Akita/miR-133aTg, and miR-133aTg mice. The green fluorescent protein represents cleaved collagen and blue color represents nuclei (DAPI staining). N = 1 per group.