Table 1.
Primer pairs (F: forward; R: reverse) used for real-time qPCR.
| Name | Pinus accession | Arabidopsis accession | e-value | Primer sequence | Functions/putative functions |
|---|---|---|---|---|---|
| Actin (ACT) |
GQ339779.1 (P. sylvestris) |
– | – | F: TGGACCTTGCTGGGCGTGATCT R: ACAATCTCGCGCTCTGCGGT |
Major component of cytoskeleton microfilaments. |
| β-Tubulin (TUB) |
KM496536.1 (P. massoniana) |
– | – | F: AAGGGGGTCAGTGTGGCAACCA R: ACAGCCCGCGGAACAAACCT |
Major component of cytoskeleton microtubules. |
| Hexokinase (HXK) |
CT580360.1 (P. pinaster) |
U28214.1 | e−77 | F: TGGCAAGGATGTGGTGGTAGCC R: TCCTCCAGCCAATGTCCCCACT |
Responsible for hexose phosphorylation. |
| Ubiquitin (UBQ) |
AF461687.1 (P. pinaster) (Sanchez et al., 2003) |
– | – | F: AGCCCTTATGCCGGAGGGGTTT R: AGTGCGGGACTCCACTGTTCCT |
Involved in protein recognition by the proteasome. |
| Ribulose 1,5-biphosphate carboxylase/oxygenase small subunit (RuBisCO) |
X13408.1 (P. thunbergii) (Yamamoto et al., 1988) |
NM_105379.4 | e−36 | F: AACCGTGGTGTCGGCGTTCA R: ACCTGCATGCATCGCACTCG |
Carbon fixation into the Calvin cycle. Also involved in photorespiration. |
| Cell wall invertase (cwINV) |
AL750756.1 (P. pinaster) |
NM_112232.4 | e−19 | F: TGGAGAAGGGGGAAAAGCGTGC R: GCAGTGACAGTGGAAGTGCCGT |
Cleaves sucrose inducing source-to-sink tissue transformation. |
| (Cytosolic) Glucose-6-phosphate dehydrogenase (G6PDH) |
CO171721.1 (P. taeda) |
AJ010970.1 | e−114 | F: AGGAACCCCATCCCAGCTGTTCA R: TCAGCCTGAGCACATTCGGG |
Responsible for the first step of the oxidative pentose phosphate pathway. |
| Pyruvate decarboxylase (PDC) |
JQ264496.1 (P. lambertiana) |
NM_124878.3 | e−11 | F: CCCGCAAACAATGACGTGGGGT R: TGCGAGCAGATGGTCCAGCA |
Involved in aerobic fermentation. |
| Glycolate oxidase (GOX) |
FN824807.1 (P. pinaster) |
AY136402.1 | e−55 | F: TGCCGGAGGTGCTGAGGATGAA R: AAAACTCGGGGGCGCAACCT |
Responsible for the first step of photorespiration. |
| Sucrose non-fermenting 1-related protein kinase 2.6 (SnRK2.6) |
DQ370129.1 (P. monticola) (Willyard et al., 2007) |
– | – | F: GGTTCATCCATGGACCTGCCAA R: TTGTCGCGCATCAACCTGGC |
Involved in the signalling of ABA-induced stomata closure, specially under drought stress. |
| Phenylalanine ammonia lyase (pal) |
AY641535.1 (P. pinaster) |
AY303128.1 | e−146 | F: TGCTGGCCACTGTGAAGCAGA R: TCGCAGAAACGGCCTGGCAA |
Involved in the synthesis of molecules crucial for plant survival under stressful scenarios, such as phenylpropanoids, flavonoids, anthocyanins, lignin, lignans, condensed tannins, and salicylic acid. |
| Thaumatin-like protein (pr5) |
JQ015859.1 (P. radiata) |
NM_106161.3 | e−17 | F: AGGAGCGCGTGTGATGCGTT R: TGAAAGTGCTGGTGGCGTCGT |
Involved in cell wall damage and formation of pores on the plasma membrane. |
| Chitinase (pr3) |
HM219849.1 (P. contorta) |
NM_112085.4 | e−70 | F: TGGCAACACGGACGCCCATT R: ACCGGCGTCGTTTCTGTGCTT |
Hydrolyzation of chitin. |
Gene names and respective GenBank accessions are indicated. Arabidopsis thaliana query sequences used for BLASTN homology search are also shown. Sequences were considered homologous for e-values lower than 10−10.