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. 2019 Apr 30;9:6654. doi: 10.1038/s41598-019-43238-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Wound healing assay of LM2 incubated with κ-carrabiose. Confluent LM2 monolayers were gently wounded and incubated in the presence of concentrations of κ-carrabiose. (A) After overnight incubation in presence of the compound (21 h) microscope photographs were taken. Controls of time 0 were carried out immediately after wounding; (B) Quantification of cell motility was carried out by measuring the distance between the invading fronts of cells in six random selected microscopic fields and the degree of motility was expressed as the Migration Index as detailed in Materials and methods. *p < 0.05, 1-way ANOVA test followed by Tukey post hoc test.