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. 2019 Apr 30;9:6646. doi: 10.1038/s41598-019-43158-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Normalized HRM plots for SPPV vaccine and other CaPVs. (a) The graphs visualize the representative profiles of normalized melt curve and (b) difference melting curve plots. Each of the genotype species were given a different colored line. The two dark columns in the melting curve plot (a) represent pre and post melting normalization regions. In the difference plot (b), SPPV field isolates have been selected as the reference for displaying the deviations between SPPV vaccine from GTPV and LSDV field isolates.