Table 2.
Cross-platform testing of the HRM assay.
| Virus Genotype | Real time PCR machines with Tm values | |||
|---|---|---|---|---|
| CFX 96 (Bio-Rad) | LC 480 II (Roche) | QS 6 (Life tech) | RG-Q (Qiagen) | |
| SPPV vaccine | 75.8–76.3 | 76.59–76.88 | 76.45–76.74 | 77.2–77.54 |
| SPPV field | 78.9–79.4 | 79.87–80.26 | 79.74–80.22 | 80.46–80.9 |
| GTPV | 80.4–80.4 | 81.39–81.52 | 81.29–81.38 | 82–82.2 |
| LSDV | 80.9–80.9 | 82.07–82.16 | 81.96–82.06 | 82.6–82.66 |
Four different real-time instruments: the LigthCycler 480 (LC 480 II), QuantStudio 6 (QS 6), RotorGene Q (RG-Q) and CFX 96 were used for assay evaluation. The respective amplicon melting temperature values are indicated for SPPV vaccine and other CaPVs.