Figure 9.

Complementation of yeast mutants on solid medium containing heavy metals. S. cerevisiae wild-type strain BY4741 was transformed with the empty vector pYES2, and mutants strains were transformed with the empty vector pYES2 or with the vectors carrying the NtMTP gene, respectively. Yeast cultures were adjusted to OD600 = 0.2, and 2 μL of serial dilutions (10-fold, from left to right in each panel) were spotted on SC-U/Gal medium supplemented with 20 mM ZnSO₄ (A), 10 mM MnSO₄ (B), 1 mM CoCl₂ (C), 60 μM CdCl₂ (D), or 5 mM FeSO₄ (E) or on the SC-U/Glu medium (control) without the supplementation. The plates were incubated for 2–4 days at 30°C. The images are representative for three independent experiments.