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. 2019 Apr 24;10:762. doi: 10.3389/fmicb.2019.00762

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Copyright © 2019 Noster, Persicke, Chao, Krone, Heppner, Hensel and Hansmeier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of the metabolic profiles of STM ΔsodAB and mutant strains defective in aconitases or fumarases and succinate dehydrogenase. STM WT and STM ΔsodAB, STM ΔacnAB, STM ΔsdhCDAB and STM ΔfumABC were cultured aerobically in LB broth for 18.5 h at 37°C. Cells were harvested and metabolites extracted for subsequent GC-MS analyses. Metabolite levels were normalized to WT levels and means of at least four biological replicates are shown. Background colors indicate the relative concentration of the respective metabolite in the mutant strains compared to WT (red = decreased; green = increased). Gray background indicates indifferent concentrations between STM mutant and WT strains (Student’s t-test, p < 0.05).